BiooTools
CRISPR design Tools

sgRNAcas9:

http://www.biootools.com/


Optimized CRISPR Design:

http://crispr.mit.edu/


CRISPR Design Tool:

http://www.broadinstitute.org/mpg/crispr_design/


Jack Lin's CRISPR/Cas9 gRNA finder:

http://spot.colorado.edu/~slin/cas9.html


E-CRISP:

http://www.e-crisp.org/E-CRISP/


fly CRISPR:

http://flycrispr.molbio.wisc.edu/


DRSC tool:

http://www.flyrnai.org/crispr/, Drosophila


ZiFiT:

http://zifit.partners.org/ZiFiT/ChoiceMenu.aspx


Cas9 Design:

http://cas9.cbi.pku.edu.cn/index.jsp


CasOT:

http://eendb.zfgenetics.org/casot/index.php


Cas-OFFinder:

http://sourceforge.net/projects/cas-offinder/


COD (Cas9 & Off-target Designer)

http://cas9.wicp.net/


Off-Spotter


DESKGEN

http://www.deskgen.com/