Package standalone

This program is free software: you can redistribute it and/or modify it under the terms of the GNU Affero General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.

This program is distributed in the hope that it will be useful,but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the GNU Affero General Public License for more details.

You should have received a copy ofthe GNU General Public License along with this program. If not, see

If you use this program in your research, please cite:

Xie et al., sgRNAcas9: a software package for designing CRISPR sgRNA and evaluating potential off-target cleavagesites.PloS one, 2014 (Link to the article)


In July, 2015, the sgRNAcas9(GUI) software v2.0,developed independently by the Huazhong Agricultural University, was certified by china state bureau of copyrights (No. 2015SR170234).

sgRNAcas9 sgRNA design source code free for academic, nonprofit, and personal use.

If you use this program in your research, please cite:

Zhao et al., Development of a graphical user interface for sgRNAcas9 and its  application. HEREDITAS(Beijing), 2015, 37(10): 1061-1072. (Link to the article)

For commercial use, please contact the author.

Please send bug reports to:ssxie\

Copyright © 2015, Huazhong Agricultural University.